Evaluate the Immune Properties of Marine-Based Collagen Dressing in Wound Care Management

A Pre-Clinical Evaluation of MariXBioHeal™ by Rozhan Vista Mehr

Author: Rozhan Vista Mehr Ltd. | Lab: Nikopharmed Arya Co Laboratory

Keywords: Collagen, Wound Healing, Fish Waste, Biomedical, Immune Properties

Introduction

In recent years, wound care products have attracted much research attention, aiming to replicate or enhance the extracellular matrix (ECM), improve patient outcomes, reduce healing time, and lower costs. Wound healing involves hemostasis, inflammation, proliferation, and remodeling. Delayed healing often results from prolonged inflammation, primarily due to elevated metalloproteinase (MMPs) activity, which degrades ECM and hinders re-epithelialization. Collagen, the most abundant ECM protein, supports fibroblast recruitment, keratinocyte migration, and tissue regeneration. Marine-derived collagen, especially from fish waste, offers a safe and cost-effective alternative for biomedical use while addressing environmental concerns. This study investigates the immune properties of marine-based collagen as a bioactive wound care component.

Methods

Marine-based collagen used in this study was provided by Rozhan Vista Mehr Ltd. Its immune properties were assessed using in-vitro cytotoxicity tests (MTT), in-vivo irritation tests, and skin sensitization studies. All assessments were conducted at Nikopharmed Arya Co Laboratory.

Results

The in-vitro cytotoxicity test on L929 mouse fibroblasts revealed 95.51% viability and no morphological reactivity after 24 hours, confirming the collagen's non-toxicity. In-vivo irritation tests in rabbits showed a negligible irritation score (0) over 72 hours, indicating the product is non-irritating. Sensitization studies in guinea pigs also recorded no visible changes, confirming the product is non-sensitizing.

Conclusion

Marine-based collagen demonstrated excellent immune compatibility, showing no signs of toxicity, irritation, or sensitization. These properties make it a promising bioactive ingredient in wound care management and other biomedical applications.